Recombinant humaan SBDS-eiwit

Ribosome maturation protein SBDS is a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The protein may function in RNA metabolism. It may be involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Shwachman-Diamond syndrome is a rare autosomal recessive disorder caused by mutations in the SBDS gene. Recombinant human SBDS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribosome maturation protein SBDS, CGI-97, SDS, SWDS

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

30.9 kDa (270aa) confirmed by MALDI-TOF

SBDS, Ribosome maturation protein SBDS, CGI-97, SDS, SWDS, ATGP0762-10 µg, ATGP0762-20 µg, ATGP0762-50 µg, ATGP0762-100 µg, ATGP0762-250 µg, ATGP0762-500 µg, ATGP0762-1 mg, ATGP0762-10, ATGP0762-20, ATGP0762-50, ATGP0762-100, ATGP0762-250, ATGP0762-500, ATGP0762-1

Tooviainen-Salo S., et al (2010) Duodecim. 126 (14) :1711-9.; ; Hesling C., et al (2007) Exp. Cell Res. 313:4180-4195

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 50 mM NaCl, 0.1mM EDTA

Metabolism

NP_057122

Q9Y3A5

Human

MSIFTPTNQI RLTNVAVVRM KRAGKRFEIA CYKNKVVGWR SGVEKDLDEV LQTHSVFVNV SKGQVAKKED LISAFGTDDQ TEICKQILTK GEVQVSDKER HTQLEQMFRD IATIVADKCV NPETKRPYTV ILIERAMKDI HYSVKTNKST KQQALEVIKQ LKEKMKIERA HMRLRFILPV NEGKKLKEKL KPLIKVIESE DYGQQLEIVC LIDPGCFREI DELIKKETKG KGSLEVLNLK DVEEGDEKFE