Recombinant humaan SDHAF2-eiwit

SDHAF2 is a mitochondrial protein needed for the flavination of a succinate dehydrogenase complex subunit required for activity of the complex. Defects in SDHAF2 are the cause of hereditary paragangliomas type 2 (PGL2), also known as familial non-chromaffin paragangliomas type 2. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. Recombinant human SDHAF2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Succinate dehydrogenase assembly factor 2 mitochondrial, Succinate dehydrogenase assembly factor 2, mitochondrial, C11orf79, PGL2, SDH5

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

19kDa (160aa) confirmed by MALDI-TOF

SDHAF2, Succinate dehydrogenase assembly factor 2 mitochondrial, Succinate dehydrogenase assembly factor 2, mitochondrial, C11orf79, PGL2, SDH5, ATGP1901-10 µg, ATGP1901-20 µg, ATGP1901-50 µg, ATGP1901-100 µg, ATGP1901-250 µg, ATGP1901-500 µg, ATGP1901-1 mg, ATGP1901-10, ATGP1901-20, ATGP1901-50, ATGP1901-100, ATGP1901-250, ATGP1901-500, ATGP1901-1

Kunst, H.P., et al. (2011) Clin. Cancer Res. 17 (2), 247-254; ; Starker, L.F., et al. (2010) Endocrine 38 (3), 397-401

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT

Metabolism

NP_060311

Q9NX18

Human

MGSSHHHHHH SSGLVPRGSH MGSSFRRFYR GDSPTDSQKD MIEIPLPPWQ ERTDESIETK RARLLYESRK RGMLENCILL SLFAKEHLQH MTEKQLNLYD RLINEPSNDW DIYYWATEAK PAPEIFENEV MALLRDFAKN KNKEQRLRAP DLEYLFEKPR