Recombinant humaan SDNSF/MCFD2-eiwit

Multiple coagulation factor deficiency 2 (MCFD2), also known as SDNSF. This is expressed by neural stem/progenitor cells of the hippocampus, and localized to region where neurogenesis persists throughout life. It has been found to prevent NSC cell death and to maintain stem cell characteristics. This protein forms a complex with LAMN1 that facilitates the transport of coagulation factors V and VIII from the endoplasmic reticulum to the Golgi apparatus via an endoplasmic reticulum Golgi intermediate compartment. Mutations in the MCFD2 gene may cause Factor V and Factor VIII combined deficiency. Recombinant human MCFD2, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Multiple coagulation factor deficiency 2, SDNSF, LMAN1IP, Multiple coagulation factor deficiency 2 MCFD 2, DKFZp686G21263, F5F8D, Neural stem cell derived neuronal survival protein, Multiple coagulation factor deficiency protein 2.

E.coli

Human

T7-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

15.1 kDa (136aa) confirmed by MALDI-TOF

SDNSF, MCFD2, Multiple coagulation factor deficiency 2, SDNSF, LMAN1IP, Multiple coagulation factor deficiency 2 MCFD 2, DKFZp686G21263, F5F8D, Neural stem cell derived neuronal survival protein, Multiple coagulation factor deficiency protein 2., ATGP0455-10 µg, ATGP0455-20 µg, ATGP0455-50 µg, ATGP0455-100 µg, ATGP0455-250 µg, ATGP0455-500 µg, ATGP0455-1 mg, ATGP0455-10, ATGP0455-20, ATGP0455-50, ATGP0455-100, ATGP0455-250, ATGP0455-500, ATGP0455-1

Zhanq B., et al. (2005) Biol Chem. 280 (27) :25881-6. ; ; Mohanty D., et al. (2005) Am J Hematol. 79:262-266. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 10% glycerol

Signal Transduction

NP_644808

Q8NI22

Human

MASMTGGQQM GRGSHMEEPA ASFSQPGSMG LDKNTVHDQE HIMEHLEGVI NKPEAEMSPQ ELQLHYFKMH DYDGNNLLDG LELSTAITHV HKEEGSEQAP LMSEDELINI IDGVLRDDDK NNDGYIDYAE FAKSLQ