Recombinant humaan SDR16C5 eiwit

Short chain dehydrogenase/reductase family 16C, member5, also known as SDR16C5, is active in both the oxidative and reductive directions. This protein oxidizes all-trans-retinol in all-trans-retinaldehyde. No activity was detected with 11-cis-retinol or 11-cis-retinaldehyde as substrates with either NAD+/NADH or NADP+/NADPH. Recombinant human SDR16C5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Short chain dehydrogenase/reductase family 16C member5, Short chain dehydrogenase/reductase family 16C, member5, RDH2, RDH-E2, RDHE2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.3 kDa (261aa) confirmed by MALDI-TOF

SDR16C5, Short chain dehydrogenase/reductase family 16C member5, Short chain dehydrogenase/reductase family 16C, member5, RDH2, RDH-E2, RDHE2, ATGP1993-10 µg, ATGP1993-20 µg, ATGP1993-50 µg, ATGP1993-100 µg, ATGP1993-250 µg, ATGP1993-500 µg, ATGP1993-1 mg, ATGP1993-10, ATGP1993-20, ATGP1993-50, ATGP1993-100, ATGP1993-250, ATGP1993-500, ATGP1993-1

Matsuzaka Y., et al. (2002) Biochem. Biophys. Res. Commun. 297:1171-1180; ; Lee S.-A., et al. (2009) Chem. Biol. Interact. 178:182-187;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_620419

Q8N3Y7

Human

MGSSHHHHHH SSGLVPRGSH MGSPKPRKNV AGEIVLITGA GSGLGRLLAL QFARLGSVLV LWDINKEGNE ETCKMAREAG ATRVHAYTCD CSQKEGVYRV ADQVKKEVGD VSILINNAGI VTGKKFLDCP DELMEKSFDV NFKAHLWTYK AFLPAMIAND HGHLVCISSS AGLSGVNGLA DYCASKFAAF GFAESVFVET FVQKQKGIKT TIVCPFFIKT GMFEGCTTGC PSLLPILEPK YAVEKIVEAI LQEKMYLYMP K