Recombinant humaan SDSL-eiwit

SDSL (serine dehydratase-like) is like L-serine dehydratase, uses pyridoxal phosphate. L-serine dehydratase, known simply as serine dehydratase (SDS), is one of three main enzymes that are involved in the metabolism of Glycine and serine. One of several members of the serine/threonine dehydratase family, SDSL may function as a serinespecific dehydratase that plays a role in protein metabolism. It has low serine dehydratase and threonine dehydratase activity. Recombinant human SDSL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Serine dehydratase-like, SDH 2, SDS-RS1, TDH

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.3 kDa (353aa) confirmed by MALDI-TOF

SDSL, Serine dehydratase-like, SDH 2, SDS-RS1, TDH, ATGP1245-10 µg, ATGP1245-20 µg, ATGP1245-50 µg, ATGP1245-100 µg, ATGP1245-250 µg, ATGP1245-500 µg, ATGP1245-1 mg, ATGP1245-10, ATGP1245-20, ATGP1245-50, ATGP1245-100, ATGP1245-250, ATGP1245-500, ATGP1245-1

Yamada T., et al. (2008) Biochim. Biophys. Acta 1780:809-818; ; Xue, H.H., et al. (1999) J. Biol. Chem. 274: 16028-16033.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl

Metabolism

NP_612441

Q96GA7

Human

MDGPVA EHAKQEPFHV VTPLLESWAL SQVAGMPVFL KCENVQPSGS FKIRGIGHFC QEMAKKGCRH LVCSSGGNAG IAAAYAARKL GIPATIVLPE STSLQVVQRL QGEGAEVQLT GKVWDEANLR AQELAKRDGW ENVPPFDHPL IWKGHASLVQ ELKAVLRTPP GALVLAVGGG GLLAGVVAGL LEVGWQHVPI IAMETHGAHC FNAAITAGKL VTLPDITSVA KSLGAKTVAA RALECMQVCK IHSEVVEDTE AVSAVQQLLD DERMLVEPAC GAALAAIYSG LLRRLQAEGC LPPSLTSVVV IVCGGNNINS RELQALKTHL GQV