Recombinant humaan SEC13 eiwit

SEC13 belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. This protein is Interacts with SEC31A and SEC31B. SEC31A was found to colocalize with SEC13, one of the other components of COPII, in the subcellular structures corresponding to the vesicle transport function. Recombinant human SEC13 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein SEC13 homolog, D3S1231E, SEC13L1, SEC13R

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

37.7 kDa (342aa) confirmed by MALDI-TOF

SEC13, Protein SEC13 homolog, D3S1231E, SEC13L1, SEC13R, ATGP1008-10 µg, ATGP1008-20 µg, ATGP1008-50 µg, ATGP1008-100 µg, ATGP1008-250 µg, ATGP1008-500 µg, ATGP1008-1 mg, ATGP1008-10, ATGP1008-20, ATGP1008-50, ATGP1008-100, ATGP1008-250, ATGP1008-500, ATGP1008-1

Stankewich M.C., et al. (2006) J. Cell Sci. 119:958-969; ; Yamasaki A., et al. (2007) Mol. Biol. Cell 17:4876-4887

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Metabolism

NP_899195

P55735

Human

MVSVINTVDT SHEDMIHDAQ MDYYGTRLAT CSSDRSVKIF DVRNGGQILI ADLRGHEGPV WQVAWAHPMY GNILASCSYD RKVIIWREEN GTWEKSHEHA GHDSSVNSVC WAPHDYGLIL ACGSSDGAIS LLTYTGEGQW EVKKINNAHT IGCNAVSWAP AVVPGSLIDH PSGQKPNYIK RFASGGCDNL IKLWKEEEDG QWKEEQKLEA HSDWVRDVAW APSIGLPTST IASCSQDGRV FIWTCDDASS NTWSPKLLHK FNDVVWHVSW SITANILAVS GGDNKVTLWK ESVDGQWVCI SDVNKGQGSV SASVTEGQQN EQ