Recombinant humaan SEC61B eiwit

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. Recombinant human SEC61B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein transport protein Sec61 subunit beta, Sec61 beta subunit

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

9.4 kDa (93aa) confirmed by MALDI-TOF

SEC61B, Protein transport protein Sec61 subunit beta, Sec61 beta subunit, ATGP2454-10 µg, ATGP2454-20 µg, ATGP2454-50 µg, ATGP2454-100 µg, ATGP2454-250 µg, ATGP2454-500 µg, ATGP2454-1 mg, ATGP2454-10, ATGP2454-20, ATGP2454-50, ATGP2454-100, ATGP2454-250, ATGP2454-500, ATGP2454-1

Bebox Z., et al. (1998) J Biol Chem. 273: 29873-29878.; ; Meyer H A., et al. (2000) J Biol Chem. 275: 14550-14557.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Signal Transduction

NP_006799

P60468

Human

MGSSHHHHHH SSGLVPRGSH MGSMPGPTPS GTNVGSSGRS PSKAVAARAA GSTVRQRKNA SCGTRSAGRT TSAGTGGMWR FYTEDSPGLK VGP