Recombinant humaan Secretaragogin eiwit

SCGN, also known as secretagogin, is a cytoplasmic protein that contains six EF-hand domains and is related to the calicium-binding proteins Calretinin and Calbindin D28K. This protein is thought to be involved in cell proliferation and KCl (potassium chloride) -mediated calcium flux events. Through its interaction with KCl and its subsequent ability to modulate calcium storage pools within the cell, SCGN may function to negatively control growth and differentiation rates and, thus, indirectly inhibit cell replication. Recombinant SCGN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Secretagogin, CALBL, DJ501N12.8, SECRET, SEGN, setagin, Secretagogin

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.2 kDa (296aa) confirmed by MALDI-TOF

Secretagogin, Secretagogin, CALBL, DJ501N12.8, SECRET, SEGN, setagin, Secretagogin, ATGP0722-10 µg, ATGP0722-20 µg, ATGP0722-50 µg, ATGP0722-100 µg, ATGP0722-250 µg, ATGP0722-500 µg, ATGP0722-1 mg, ATGP0722-10, ATGP0722-20, ATGP0722-50, ATGP0722-100, ATGP0722-250, ATGP0722-500, ATGP0722-1

Wagner L., et al. (2000) J Biol Chem. 275 (32) :24740-51. ; ; Gartner W., et al. (2001) Cereb Cortex. 11 (12) :1161-9.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Neuroscience

NP_008929

O76038

Human

MDSSREPTLG RLDAAGFWQV WQRFDADEKG YIEEKELDAF FLHMLMKLGT DDTVMKANLH KVKQQFMTTQ DASKDGRIRM KELAGMFLSE DENFLLLFRR ENPLDSSVEF MQIWRKYDAD SSGFISAAEL RNFLRDLFLH HKKAISEAKL EEYTGTMMKI FDRNKDGRLD LNDLARILAL QENFLLQFKM DACSTEERKR DFEKIFAYYD VSKTGALEGP EVDGFVKDMM ELVQPSISGV DLDKFREILL RHCDVNKDGK IQKSELALCL GLKINP