Recombinant humaan Selenoproteïne R/MSRB1-eiwit

SEPX1, also known as Methionine sulfoxide reductase B1 (MSRB1), is a selenoprotein. Methionine sulfoxide reductases (MSRs) catalyze reduction of free and protein-bound methionine sulfoxides to corresponding methionines. The oxidation of methionine by ROS generates a diastereomeric mixture of methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO) . Two distinct enzyme families evolved for reduction of these sulfoxides, with methionine-S-sulfoxide reductase (MsrA) being stereospecific for Met-S-SO and methionine-R-sulfoxide reductase (MsrB) for Met-R-SO. In bacteria, the selenocystein (Sec/u) element is located immediately following the uGA codon within the reading frame for the selenoprotein so we mutated Sec-95 to Cys. Recombinant human SEPX1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

HSPC270, Methionine-R-sulfoxide reductase B1, MsrB1, Selenoprotein X, SELENOR, SELENOX, SelR, SelX, SepR, SEPX1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

14.8 kDa (136aa) confirmed by MALDI-TOF

SEPX1, Methionine-R-sulfoxide reductase B1, Selenoprotein X, HSPC270, MSRB1, SELR, SELX, MSRB1, Methionine-R-sulfoxide reductase B1, u95C, ATGP0735-10 µg, ATGP0735-20 µg, ATGP0735-50 µg, ATGP0735-100 µg, ATGP0735-250 µg, ATGP0735-500 µg, ATGP0735-1 mg, ATGP0735-10, ATGP0735-20, ATGP0735-50, ATGP0735-100, ATGP0735-250, ATGP0735-500, ATGP0735-1

Gladdyshev VN., et al. (2010) PLoS One. 5 (7) :e11497.; ; Thiele JJ., et al. (2009) Am J Dermatopathol. 31 (5) :427-31.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 0.1mM PMSF, 2mM EDTA, 10% glycerol

Redox Proteins

NP_057416

Q9NZV6

Human

MSFCSFFGGE VFQNHFEPGV YVCAKCGYEL FSSRSKYAHS SPWPAFTETI HADSVAKRPE HNRSEALKVS CGKCGNGLGH EFLNDGPKPG QSRFCIFSSS LKFVPKGKET SASQGH