Recombinant humaan SEMG1-eiwit

SEMG1 is the predominant protein in semen. The secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human SEMG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Semenogelin-1, Semenogelin 1, CT103, dJ172H20.2, SEMG, SGI

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

52 kDa (462aa)

SEMG1, Semenogelin-1, Semenogelin 1, CT103, dJ172H20.2, SEMG, SGI, ATGP2151-10 µg, ATGP2151-20 µg, ATGP2151-50 µg, ATGP2151-100 µg, ATGP2151-250 µg, ATGP2151-500 µg, ATGP2151-1 mg, ATGP2151-10, ATGP2151-20, ATGP2151-50, ATGP2151-100, ATGP2151-250, ATGP2151-500, ATGP2151-1

Brillard-Bourdet M, et al. (2002) . Eur J Biochem. 269 (1) :390-5

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.5M NaCl, 10% glycerol, 250 mM Imidazole, 0.1mM PMSF, 1mM DTT

Signal Transduction

NP_002998

P04279

Human

MGSSHHHHHH SSGLVPRGSH MGSQKGGSKG RLPSEFSQFP HGQKGQHYSG QKGKQQTESK GSFSIQYTYH VDANDHDQSR KSQQYDLNAL HKTTKSQRHL GGSQQLLHNK QEGRDHDKSK GHFHRVVIHH KGGKAHRGTQ NPSQDQGNSP SGKGISSQYS NTEERLWVHG LSKEQTSVSG AQKGRKQGGS QSSYVLQTEE LVANKQQRET KNSHQNKGHY QNVVEVREEH SSKVQTSLCP AHQDKLQHGS KDIFSTQDEL LVYNKNQHQT KNLNQDQQHG RKANKISYQS SSTEERRLHY GENGVQKDVS QSSIYSQTEE KAQGKSQKQI TIPSQEQEHS QKANKISYQS SSTEERRLHY GENGVQKDVS QRSIYSQTEK LVAGKSQIQA PNPKQEPWHG ENAKGESGQS TNREQDLLSH EQKGRHQHGS HGGLDIVIIE QEDDSDRHLA QHLNNDRNPL FT