Recombinant humaan sepiapterinereductase/SPR-eiwit

Sepiapterin reductase (SPR) belongs to the short-chain dehydrogenase/reductase (SDR) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. SPR is an essential enzyme for the biosynthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydrolases including tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Defects in SPR cause DOPA-responsive dystonia defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Recombinant human SPR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

SPR, Sepiapterin reductase, SDR38C1, Short chain dehydrogenase/reductase family 38C, member 1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

30.2 kDa (281aa) confirmed by MALDI-TOF

Sepiapterin reductase, SPR, Sepiapterin reductase., ATGP0288-10 µg, ATGP0288-20 µg, ATGP0288-50 µg, ATGP0288-100 µg, ATGP0288-250 µg, ATGP0288-500 µg, ATGP0288-1 mg, ATGP0288-10, ATGP0288-20, ATGP0288-50, ATGP0288-100, ATGP0288-250, ATGP0288-500, ATGP0288-1

Tobin JE., et al. (2007) . Brain Res. 30:42-7.; ; Ohye T., et al. (1998) . Biochem Biophys Res Commun. 251 (2) :597-602.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) 10% glycerol

Redox Proteins

NP_003115

P35270

Human

MGSSHHHHHH SSGLVPRGSH MEGGLGRAVC LLTGASRGFG RTLAPLLASL LSPGSVLVLS ARNDEALRQL EAELGAERSG LRVVRVPADL GAEAGLQQLL GALRELPRPK GLQRLLLINN AGSLGDVSKG FVDLSDSTQV NNYWALNLTS MLCLTSSVLK AFPDSPGLNR TVVNISSLCA LQPFKGWALY CAGKAARDML FQVLALEEPN VRVLNYAPGP LDTDMQQLAR ETSVDPDMRK GLQELKAKGK LVDCKVSAQK LLSLLEKDEF KSGAHVDFYD K