Recombinant humaan SEPSECS-eiwit

SEPSECS, also known as SLA/LP autoantigen, catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA (sec) to selenocysteinyl-tRNA (sec) using selenophosphate as the selenium donor. Also, this protein is considered a specific marker of autoimmune hepatitis. Recombinant human SEPSECS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

O-phosphoseryl-tRNA (Sec) selenium transferase, Liver-pancreas antigen, LP, SLA-p35, SLA/LP autoantigen, Selenocysteine synthase, Sec synthase, Selenocysteinyl-tRNA (Sec) synthase, Sep-tRNA:Sec-tRNA synthase, SepSecS, Soluble liver antigen, SLA, UGA suppressor tRNA-associated protein, tRNA (Ser/Sec) -associated antigenic protein, TRNP48

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

57.9 kDa (521aa) confirmed by MALDI-TOF

Liver-pancreas antigen, LP, O-phosphoseryl-tRNA (Sec) selenium transferase, Sec synthase, Selenocysteine synthase, Selenocysteinyl-tRNA (Sec) synthase, SepSecS, Sep-tRNA:Sec-tRNA synthase, SLA, SLA/LP autoantigen, SLA-p35, Soluble liver antigen, tRNA (Ser/Sec) -associated antigenic protein, TRNP48, UGA suppressor tRNA-associated protein, ATGP0806-10 µg, ATGP0806-20 µg, ATGP0806-50 µg, ATGP0806-100 µg, ATGP0806-250 µg, ATGP0806-500 µg, ATGP0806-1 mg, ATGP0806-010, ATGP0806-020, ATGP0806-050, ATGP0806-100, ATGP0806-250, ATGP0806-500, ATGP0806-01M

Palioura S., et al. (2009) Science. 325 (5938) :321-5.; ; Yuan J., et al. (2006) Proc. Natl. Acad. Sci. u.S.A. 103:18923-18927

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Enzymes & Proteases

NP_058651.3

Q9HD40

Human

MNRESFAAGE RLVSPAYVRQ GCEARRSHEH LIRLLLEKGK CPENGWDEST LELFLHELAI MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD ISAVQPKAAG SSLLNKITNS LVLDIIKLAG VHTVANCFVV PMATGMSLTL CFLTLRHKRP KAKYIIWPRI DQKSCFKSMI TAGFEPVVIE NVLEGDELRT DLKAVEAKVQ ELGPDCILCI HSTTSCFAPR VPDRLEELAV ICANYDIPHI VNNAYGVQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFND SFIQEISKMY PGRASASPSL DVLITLLSLG SNGYKKLLKE RKEMFSYLSN QIKKLSEAYN ERLLHTPHNP ISLAMTLKTL DEHRDKAVTQ LGSMLFTRQV SGARVVPLGS MQTVSGYTFR GFMSHTNNYP CAYLNAASAI GMKMQDVDLF IKRLDRCLKA VRKERSKESD DNYDKTEDVD IEEMALKLDN VLLDTYQDAS S