Recombinant humaan SEPT2/SEPTIN2-eiwit

Septin2 (SEPT2) is a GTPase that is required for cytokinesis and associated with exocytosis. This protein can hetero-oligomerize with Septin6, 7 and also plays a role in the organization of new growth in organisms. It is associated with a Tau-based paired helical filament core and may contribute to the formation of neurofibrillary tangle as integral constituents of paired helical filaments. Recombinant human Septin2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Septin 2, hNedd5, KIAA0158, DIFF6, Pnutl3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

43.6 kDa (381aa) confirmed by MALDI-TOF

SETP 2, SETP-2, Septin-2, Septin 2, hNedd5, KIAA0158, DIFF6, Pnutl3, ATGP0717-10 µg, ATGP0717-20 µg, ATGP0717-50 µg, ATGP0717-100 µg, ATGP0717-250 µg, ATGP0717-500 µg, ATGP0717-1 mg, ATGP0717-010, ATGP0717-020, ATGP0717-050, ATGP0717-100, ATGP0717-250, ATGP0717-500, ATGP0717-01M

Low C, et al.. (2006) J Biol Chem. 281 (41) :30697-706.; ; Kremer BE, et al. (2007) Cell. 130 (5) :837-50.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl,1mM DTT

Cell Cycle

AAH14455

Q15019

Human

MSKQQPTQFI NPETPGYVGF ANLPNQVHRK SVKKGFEFTL MVVGESGLGK STLINSLFLT DLYPERVISG AAEKIERTVQ IEASTVEIEE RGVKLRLTVV DTPGYGDAIN CRDCFKTIIS YIDEQFERYL HDESGLNRRH IIDNRVHCCF YFISPFGHGL KPLDVAFMKA IHNKVNIVPV IAKADTLTLK ERERLKKRIL DEIEEHNIKI YHLPDAESDE DEDFKEQTRL LKASIPFSVV GSNQLIEAKG KKVRGRLYPW GVVEVENPEH NDFLKLRTML ITHMQDLQEV TQDLHYENFR SERLKRGGRK VENEDMNKDQ ILLEKEAELR RMQEMIARMQ AQMQMQMQGG DGDGGALGHH V