Recombinant humaan SEPT3/SEPTIN3-eiwit

SEPT3 belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms. Recombinant human SEPT3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Septin 3, Neuronal-specific septin-3, 41885

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BRADFORD assay)

> 90% by SDS-PAGE

43.1 kDa (381aa) confirmed by MALDI-TOF

SETP 3, SETP-3, Septin-3, Septin 3, Neuronal-specific septin-3, 41885, ATGP2919-10 µg, ATGP2919-20 µg, ATGP2919-50 µg, ATGP2919-100 µg, ATGP2919-250 µg, ATGP2919-500 µg, ATGP2919-1 mg, ATGP2919-010, ATGP2919-020, ATGP2919-050, ATGP2919-100, ATGP2919-250, ATGP2919-500, ATGP2919-01M

Methner A. et al. (2001) Biochem. Biophys. Res. Commun. 283:48-56.; ; Macedo J.N. et al. (2013) Biochem. J. 450:95-105.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol 1mM DTT

Cell Cycle

NP_663786.2

Q9UH03

Human

MSKGLPE TRTDAAMSEL VPEPRPKPAV PMKPMSINSN LLGYIGIDTI IEQMRKKTMK TGFDFNIMVV GQSGLGKSTL VNTLFKSQVS RKASSWNREE KIPKTVEIKA IGHVIEEGGV KMKLTVIDTP GFGDQINNEN CWEPIEKYIN EQYEKFLKEE VNIARKKRIP DTRVHCCLYF ISPTGHSLRP LDLEFMKHLS KVVNIIPVIA KADTMTLEEK SEFKQRVRKE LEVNGIEFYP QKEFDEDLED KTENDKIRQE SMPFAVVGSD KEYQVNGKRV LGRKTPWGII EVENLNHCEF ALLRDFVIRT HLQDLKEVTH NIHYETYRAK RLNDNGGLPP GEGLLGTVLP PVPATPCPTA E