Recombinant humaan SEPT5/SEPTIN5 eiwit

SEPT5, also known as septin-5, belongs to the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Recombinant human SEPT5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Septin 5, Peanut-like proein 1, PNUTL1, HCDCREL-1, H5, Cell division control-related protein 1, CDCrel-1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.2 kDa (392aa) confirmed by MALDI-TOF

Septin-5, Septin 5, Peanut-like proein 1, PNUTL1, HCDCREL-1, H5, Cell division control-related protein 1, SEPT 5, SEPT-5, CDCREL, CDCREL-1, CDCREL1, ATGP1121-10 µg, ATGP1121-20 µg, ATGP1121-50 µg, ATGP1121-100 µg, ATGP1121-250 µg, ATGP1121-500 µg, ATGP1121-1 mg, ATGP1121-010, ATGP1121-020, ATGP1121-050, ATGP1121-100, ATGP1121-250, ATGP1121-500, ATGP1121-01M

Blaeser S., et al. (2002) FEBS Lett. 519:169-172; ; Hall P.A., et al. (2005) J. Pathol. 206:269-278

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM MES buffer (pH 5.0) containing 10% glycerol, 0.5M NaCl, 250 mM Imidazole, 0.1mM PMSF

Signal Transduction

NP_002679.2

Q99719

Human

MSTGLRY KSKLATPEDK QDIDKQYVGF ATLPNQVHRK SVKKGFDFTL MVAGESGLGK STLVHSLFLT DLYKDRKLLS AEERISQTVE ILKHTVDIEE KGVKLKLTIV DTPGFGDAVN NTECWKPITD YVDQQFEQYF RDESGLNRKN IQDNRVHCCL YFISPFGHGL RPVDVGFMKA LHEKVNIVPL IAKADCLVPS EIRKLKERIR EEIDKFGIHV YQFPECDSDE DEDFKQQDRE LKESAPFAVI GSNTVVEAKG QRVRGRLYPW GIVEVENQAH CDFVKLRNML IRTHMHDLKD VTCDVHYENY RAHCIQQMTS KLTQDSRMES PIPILPLPTP DAETEKLIRM KDEELRRMQE MLQRMKQQMQ DQ