Recombinant humaan SEPT6/SEPTIN6-eiwit

Septins belong to the GTPase superclass of proteins and have been functionally implicated in cytokinesis and the maintenance of cellular morphology. SEPT6 is member of septin family and interact with SEPT2. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. Recombinant human SEPT6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Septin 6, 40788

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

52.1 kDa, (457aa)

SEPT 6, SEPT-6, Septin-6, Septin 6, 40788, ATGP1244-10 µg, ATGP1244-20 µg, ATGP1244-50 µg, ATGP1244-100 µg, ATGP1244-250 µg, ATGP1244-500 µg, ATGP1244-1 mg, ATGP1244-010, ATGP1244-020, ATGP1244-050, ATGP1244-100, ATGP1244-250, ATGP1244-500, ATGP1244-01M

Nakahira M, et al. (2010) PLoS One. 5 (11) :e13799; ; Kremer B.E., et al. (2007) Cell 130:837-850

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl

Cell Cycle

AAH09291

Q14141

Human

MGSSHHHHHH SSGLVPRGSH MGSMAATDIA RQVGEGCRTV PLAGHVGFDS LPDQLVNKSV SQGFCFNILC VGETGLGKST LMDTLFNTKF EGEPATHTQP GVQLQSNTYD LQESNVRLKL TIVSTVGFGD QINKEDSYKP IVEFIDAQFE AYLQEELKIR RVLHTYHDSR IHVCLYFIAP TGHSLKSLDL VTMKKLDSKV NIIPIIAKAD AISKSELTKF KIKITSELVS NGVQIYQFPT DDESVAEING TMNAHLPFAV IGSTEELKIG NKMMRARQYP WGTVQVENEA HCDFVKLREM LIRVNMEDLR EQTHTRHYEL YRRCKLEEMG FKDTDPDSKP FSLQETYEAK RNEFLGELQK KEEEMRQMFV QRVKEKEAEL KEAEKELHEK FDRLKKLHQD EKKKLEDKKK SLDDEVNAFK QRKTAAELPQ SQGSQAGGSQ TLKRDKEKKN NPWLCTE