Recombinant humaan serglycine/SRGN-eiwit

SRGN is a protein best known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of many hematopoietic cells also contain a protease-resistant peptide core, which may be important for neutralizing hydrolytic enzymes. This protein was found to be associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. Two transcript variants, only one of them protein-coding, have been found for this gene. Recombinant human SRGN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Serglycin precursor, FLJ12930, MGC9289, PPG, PRG, PRG1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17.4 kDa (156aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

SRGN, Serglycin precursor, FLJ12930, MGC9289, PPG, PRG, PRG1, ATGP1242-10 µg, ATGP1242-20 µg, ATGP1242-50 µg, ATGP1242-100 µg, ATGP1242-250 µg, ATGP1242-500 µg, ATGP1242-1 mg, ATGP1242-10, ATGP1242-20, ATGP1242-50, ATGP1242-100, ATGP1242-250, ATGP1242-500, ATGP1242-1

Niemann Cu., et al. (2004) J Leukoc Biol. 76 (2) :406-15.; ; Raja SM., et al. (2000) J Biol Chem. 277 (51) :49523-30.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.

Cytoskeleton

NP_002718

P10124

Human

MGSSHHHHHH SSGLVPRGSH MGSHMYPTRR ARYQWVRCNP DSNSANCLEE KGPMFELLPG ESNKIPRLRT DLFPKTRIQD LNRIFPLSED YSGSGFGSGS GSGSGSGSGF LTEMEQDYQL VDESDAFHDN LRSLDRNLPS DSQDLGQHGL EEDFML