Recombinant humaan Serpin B5/maspin-eiwit

Serpin B5/Maspin, also known as serpin family B member 5, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion. Also, it is present in normal breast and prostatic epithelial cells but down regulated in the respective carcinomas. Recombinant human Serpin B5/Maspin, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Acrosomal serine protease inhibitor, IPSP, IPSP_HUMAN, PAI 3, PAI-3, PAI3, PCI, PCI-B, PLANH 3, PLANH3, Plasma serine protease inhibitor, Plasminogen activator inhibitor 3, Plasminogen activator inhibitor III, PROCI, Protein C inhibitor, Serpin A5, Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 5, Serpin peptidase inhibitor clade A member 5, Serpina5

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

44.2 kDa (395aa) confirmed by MALDI-TOF

Serpin B5, Maspin, ATGP3868-10 µg, ATGP3868-20 µg, ATGP3868-50 µg, ATGP3868-100 µg, ATGP3868-250 µg, ATGP3868-500 µg, ATGP3868-1 mg, ATGP3868-10, ATGP3868-20, ATGP3868-50, ATGP3868-100, ATGP3868-250, ATGP3868-500, ATGP3868-1

Lockett J., et al. (2006) J Cell Biochem. 97 (4) :651-60.; ; Khalkhali Ellis Z., et al. (2006) Clin Cancer Res. 12 (24) :7279-83.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Cancer

NP_002630

P36952

Human

MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDVPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFSESET KECPFRVNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP