Recombinant humaan Serpin B8/proteinaseremmer 8 eiwit

The superfamily of high molecular weight serine proteinase inhibitors (serpins) regulate a diverse set of intracellular and extracellular processes such as complement activation, fibrinolysis, coagulation, cellular differentiation, tumor suppression, apoptosis, and cell migration. SERPINB8 is a member of the ov-serpin subfamily, which, relative to the archetypal serpin PI1, is characterized by a high degree of homology to chicken ovalbumin, lack of N- and C-terminal extensions, absence of a signal peptide, and a serine rather than an asparagine residue at the penultimate position. Recombinant human SERPINB8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Serpin B8, Serpin B8, CAP2, PI8

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

45.2 kDa (397aa) confirmed by MALDI-TOF

Serpin B8, Proteinase Inhibitor8, Serpin B8, Serpin B8, CAP2, PI8, ATGP2909-10 µg, ATGP2909-20 µg, ATGP2909-50 µg, ATGP2909-100 µg, ATGP2909-250 µg, ATGP2909-500 µg, ATGP2909-1 mg, ATGP2909-10, ATGP2909-20, ATGP2909-50, ATGP2909-100, ATGP2909-250, ATGP2909-500, ATGP2909-1

Sprecher C.A., et al. (1995) J. Biol. Chem. 270:29854-29861.; ; Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Cardiovascular

NP_942130

P50452

Human

MDDLCEA NGTFAISLFK ILGEEDNSRN VFFSPMSISS ALAMVFMGAK GSTAAQMSQA LCLYKDGDIH RGFQSLLSEV NRTGTQYLLR TANRLFGEKT CDFLPDFKEY CQKFYQAELE ELSFAEDTEE CRKHINDWVA EKTEGKISEV LDAGTVDPLT KLVLVNAIYF KGKWNEQFDR KYTRGMLFKT NEEKKTVQMM FKEAKFKMGY ADEVHTQVLE LPYVEEELSM VILLPDDNTD LAVVEKALTY EKFKAWTNSE KLTKSKVQVF LPRLKLEESY DLEPFLRRLG MIDAFDEAKA DFSGMSTEKN VPLSKVAHKC FVEVNEEGTE AAAATAVVRN SRCSRMEPRF CADHPFLFFI RHHKTNCILF CGRFSSP