Recombinant humaan Serpin C1/Antitrombine-III eiwit

SERPINC1, also known as antithrombin-III, is a member of the Serpin superfamily of the serine protease inhibitors. It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa. It is one of the most important coagulation inhibitors and is the basic enzyme for the therapeutic action of heparin. In common with SerpinA5 and D1, the inhibitory activity of SerpinC1 dramatically increases the presence of heparin and other glycosaminoglycans. It mediates the promotion of prostaglandin release, inhibitors of leukocyte activation and down-regulation of many inflammatory cytokines. This protein has anti-inflammatory properties besides anticoagulant. Recombinant human SERPINC1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 1nM.

Antithrombin-III, SERPINC1, AT3, AT3D, ATIII, THPH7

Baculovirus

Human

His-Tag

Enzyme Activity, SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 1nM.

50.1 kDa (441aa)

Serpin C1, Antithrombin-2I, Antithrombin-III, SERPINC1, AT3, AT3D, ATIII, THPH7, ATGP3791-10 µg, ATGP3791-20 µg, ATGP3791-50 µg, ATGP3791-100 µg, ATGP3791-250 µg, ATGP3791-500 µg, ATGP3791-1 mg, ATGP3791-10, ATGP3791-20, ATGP3791-50, ATGP3791-100, ATGP3791-250, ATGP3791-500, ATGP3791-1

Caglikulekci M., et al. (2004) Ann Chir. 129:273-277.; ; Chuang, Y.-J., et al. (2001) Biochemistry 40:6670-6679.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_000479

P01008

Human

Ala

ADPHGSPVDI CTAKPRDIPM NPMCIYRSPE KKATEDEGSE QKIPEATNRR VWELSKANSR FATTFYQHLA DSKNDNDNIF LSPLSISTAF AMTKLGACND TLQQLMEVFK FDTISEKTSD QIHFFFAKLN CRLYRKANKS SKLVSANRLF GDKSLTFNET YQDISELVYG AKLQPLDFKE NAEQSRAAIN KWVSNKTEGR ITDVIPSEAI NELTVLVLVN TIYFKGLWKS KFSPENTRKE LFYKADGESC SASMMYQEGK FRYRRVAEGT QVLELPFKGD DITMVLILPK PEKSLAKVEK ELTPEVLQEW LDELEEMMLV VHMPRFRIED GFSLKEQLQD MGLVDLFSPE KSKLPGIVAE GRDDLYVSDA FHKAFLEVNE EGSEAAASTA VVIAGRSLNP NRVTFKANRP FLVFIREVPL NTIIFMGRVA NPCVKHHHHH H