Recombinant humaan SERTAD1-eiwit

SERTAD1, also known as SERTA domain-containing protein, acts at E2F-responsive promoters to integrate signals provided by PHD- and/or bromodomain-containing transcription factors. This protein stimulates E2F-1/DP-1 transcriptional activity. Recombinant human SERTAD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

SERTA domain-containing protein, SEI1, TRIP-Br1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

27.3 kDa (260aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

SERTAD1, SERTA domain-containing protein, SEI1, TRIP-Br1, ATGP1638-10 µg, ATGP1638-20 µg, ATGP1638-50 µg, ATGP1638-100 µg, ATGP1638-250 µg, ATGP1638-500 µg, ATGP1638-1 mg, ATGP1638-10, ATGP1638-20, ATGP1638-50, ATGP1638-100, ATGP1638-250, ATGP1638-500, ATGP1638-1

Hsu S.-I., et al. (2001) EMBO J. 20:2273-2285; ; Sugimoto M., et al. (1999) Genes Dev. 13:3027-3033

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_037508.2

Q9UHV2

Human

MLSKGL KRKREEEEEK EPLAVDSWWL DPGHTAVAQA PPAVASSSLF DLSVLKLHHS LQQSEPDLRH LVLVVNTLRR IQASMAPAAA LPPVPSPPAA PSVADNLLAS SDAALSASMA SLLEDLSHIE GLSQAPQPLA DEGPPGRSIG GAAPSLGALD LLGPATGCLL DDGLEGLFED IDTSMYDNEL WAPASEGLKP GPEDGPGKEE APELDEAELD YLMDVLVGTQ ALERPPGPGR