Recombinant humaan SET-eiwit

SET inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT) . This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. Recombinant human SET protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein SET isoform 1, 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

35.9 kDa (313aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

SET, Protein SET isoform 1, 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA, ATGP2191-10 µg, ATGP2191-20 µg, ATGP2191-50 µg, ATGP2191-100 µg, ATGP2191-250 µg, ATGP2191-500 µg, ATGP2191-1 mg, ATGP2191-10, ATGP2191-20, ATGP2191-50, ATGP2191-100, ATGP2191-250, ATGP2191-500, ATGP2191-1

Karetsou Z, Emmanouilidou A, et al. (2009) . BMC Biochem. 10:10. ; ; Li M, Makkinje A, et al. (1996) . J Biol Chem. 271 (19) :11059-62.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_001116293

Q01105

Human

MGSSHHHHHH SSGLVPRGSH MGSMAPKRQS PLPPQKKKPR PPPALGPEET SASAGLPKKG EKEQQEAIEH IDEVQNEIDR LNEQASEEIL KVEQKYNKLR QPFFQKRSEL IAKIPNFWVT TFVNHPQVSA LLGEEDEEAL HYLTRVEVTE FEDIKSGYRI DFYFDENPYF ENKVLSKEFH LNESGDPSSK STEIKWKSGK DLTKRSSQTQ NKASRKRQHE EPESFFTWFT DHSDAGADEL GEVIKDDIWP NPLQYYLVPD MDDEEGEGEE DDDDDEEEEG LEDIDEEGDE DEGEEDEDDD EGEEGEEDEG EDD