Recombinant humaan SGT1/SUGT1-eiwit

SuGT1, also known as suppressor of G2 allele of SKP1, is a homolog of the yeast protein SGT1, a regulator of the cell cycle that is essential for G1/S and G2/M transitions. It contains a CS domain, a SGS domain, a p23 domain and three tetratricopeptide repeats (TPR) . This protein associates with Skp1 p19 and CuL-1, subunits of the SCF ubiquitin ligase complex, and is thought to play a role in protein degradation. In addition, it is required for the kinetochores assembly, and has function as a co-chaperone for HSP90. Recombinant human SuGT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Suppressor of G2 allele of SKP1 homolog isoform SGT1B, SGT1, SGT1 homolog, MIS12 kinetochore complex assembly cochaperone, Protein SGT1 homolog, Protein 40-6-3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.7 kDa (272aa) confirmed by MALDI-TOF

MIS12 kinetochore complex assembly cochaperone, Protein 40-6-3, Protein SGT1 homolog, SGT1, SGT1 homolog, SUGT1, Suppressor of G2 allele of SKP1 homolog isoform SGT1B, ATGP0899-10 µg, ATGP0899-20 µg, ATGP0899-50 µg, ATGP0899-100 µg, ATGP0899-250 µg, ATGP0899-500 µg, ATGP0899-1 mg, ATGP0899-010, ATGP0899-020, ATGP0899-050, ATGP0899-100, ATGP0899-250, ATGP0899-500, ATGP0899-01M

Spiechowicz M., et al. (2006) . Neurochem. Int. 49: 487-493.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT, 0.1M NaCl

Cell Cycle

NP_001124384

Q9Y2Z0

Human

HRVGQAGLQ LLTSSDPPAL DSQSAGITGA DANFSVWIKR CQEAQNGSES EVWTHQSKIK YDWYQTESQV VITLMIKNVQ KNDVNVEFSE KELSALVKLP SGEDYNLKLE LLHPIIPEQS TFKVLSTKIE IKLKKPEAVR WEKLEGQGDV PTPKQFVADV KNLYPSSSPY TRNWDKLVGE IKEEEKNEKL EGDAALNRLF QQIYSDGSDE VKRAMNKSFM ESGGTVLSTN WSDVGKRKVE INPPDDMEWK KY