Recombinant humaan SGTA-eiwit

SGTA, also known as SGT or hSGT, is a ubiquitously expressed protein that contains three TPR protein-protein interaction repeats. This protien is believed to function as a component of the androgen receptor (AR) -chaperone-cochaperone complex, acting as a cochaperone involved in androgen signaling. In addition, SGTA functions as a cochaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity. On the basis of its role in apoptosis and androgen signaling, SGTA is a potential candidate for PCOS (polycystic ovary syndrome), a disorder characterized by androgen excess, obesity and menstrual disturbances. Recombinant SGTA protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Small glutamine-rich tetratricopeptide repeat-containing protein alpha, alphaSGT, hSGT, SGT, Small glutamine-rich tetratricopeptide repeat-containing protein alpha Small glutamine rich tetratricopeptide repeat TPR containing alpha, uBP, Vpu binding protein.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.1 kDa (321aa) confirmed by MALDI-TOF

SGTA, Small glutamine-rich tetratricopeptide repeat-containing protein alpha, alphaSGT, hSGT, SGT, Small glutamine-rich tetratricopeptide repeat-containing protein alpha Small glutamine rich tetratricopeptide repeat TPR containing alpha, uBP, Vpu binding protein., ATGP0533-10 µg, ATGP0533-20 µg, ATGP0533-50 µg, ATGP0533-100 µg, ATGP0533-250 µg, ATGP0533-500 µg, ATGP0533-1 mg, ATGP0533-10, ATGP0533-20, ATGP0533-50, ATGP0533-100, ATGP0533-250, ATGP0533-500, ATGP0533-1

Cziepluch C., et al. (1998) J Virol. 72 (5) :4149-56. ; ; Callahan MA., et al. (1998) J Virol. 72 (6) :5189-97. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Heat Shock Proteins

NP_003012

O43765

Human

MDNKKRLAYA IIQFLHDQLR HGGLSSDAQE SLEVAIQCLE TAFGVTVEDS DLALPQTLPE IFEAAATGKE MPQDLRSPAR TPPSEEDSAE AERLKTEGNE QMKVENFEAA VHFYGKAIEL NPANAVYFCN RAAAYSKLGN YAGAVQDCER AICIDPAYSK AYGRMGLALS SLNKHVEAVA YYKKALELDP DNETYKSNLK IAELKLREAP SPTGGVGSFD IAGLLNNPGF MSMASNLMNN PQIQQLMSGM ISGGNNPLGT PGTSPSQNDL ASLIQAGQQF AQQMQQQNPE LIEQLRSQIR SRTPSASNDD QQELEHHHHH H