Recombinant humaan SH3BGRL-eiwit

SH3BGRL, also known as SH3 domain-binding glutamic acid-rich-like protein, is a member of the human SH3BGR family. SH3BGRL gene located to chromosome Xq13. 3 encodes for a small protein of 114 amino acids, which is apparently widely expressed in many tissues including liver and blood. SH3BGRL protein features a prolinerich sequence (PLPPQIF), which contains both the SH3 binding (PXXP) 3 and the Homer EVH1 binding (PPXXF) 4 motif. This proline-rich region is highly conserved in human SH3BGR family and was expected to be functionally important. Recombinant human SH3BGRL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

SH3 domain-binding glutamic acid-rich-like protein, SH3BGR

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

15.3 kDa (138aa) confirmed by MALDI-TOF

SH3BGRL, SH3 domain-binding glutamic acid-rich-like protein, SH3BGR, ATGP1640-10 µg, ATGP1640-20 µg, ATGP1640-50 µg, ATGP1640-100 µg, ATGP1640-250 µg, ATGP1640-500 µg, ATGP1640-1 mg, ATGP1640-10, ATGP1640-20, ATGP1640-50, ATGP1640-100, ATGP1640-250, ATGP1640-500, ATGP1640-1

Egeo A., et al. (1998) Biochem. Biophys. Res. Commun. 247:302-306; ; Yin L., et al. (2006) Proteins. 61:213-216

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Signal Transduction

NP_003013

O75368

Human

MGSSHHHHHH SSGLVPRGSH MGSHMVIRVY IASSSGSTAI KKKQQDVLGF LEANKIGFEE KDIAANEENR KWMRENVPEN SRPATGYPLP PQIFNESQYR GDYDAFFEAR ENNAVYAFLG LTAPPGSKEA EVQAKQQA