Recombinant humaan SH3BGRL3-eiwit

SH3 domain-binding glutamic acid-rich-like protein 3, also known as SH3BGRL3, is located to chromosome 1p34. 3-35 encodes for a small protein of 93 amino acids. SH3BGRL3 is the new member of thioredoxin (TRX) super family, whose posttranslational modified form was identified as tumor necrosis factor alpha (TNF-alpha) inhibitory protein. It may function as a regulator in all-trans retinoic acid-induced pathway. Recombinant human SH3BGRL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

SH3 domain-binding glutamic acid-rich-like protein 3, SH3 domain-binding glutamic acid-rich-like protein 3, SH3BP-1, TIP-B1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

12.8 kDa (116aa) confirmed by MALDI-TOF

SH3BGRL3, SH3 domain-binding glutamic acid-rich-like protein 3, SH3 domain-binding glutamic acid-rich-like protein 3, SH3BP-1, TIP-B1, ATGP1715-10 µg, ATGP1715-20 µg, ATGP1715-50 µg, ATGP1715-100 µg, ATGP1715-250 µg, ATGP1715-500 µg, ATGP1715-1 mg, ATGP1715-10, ATGP1715-20, ATGP1715-50, ATGP1715-100, ATGP1715-250, ATGP1715-500, ATGP1715-1

Mazzocco M., et al. (2002) Gene. 291:233-9; ; Xu C., et al. (2005) FEBS Lett. 579: 2788-94.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT

Redox Proteins

NP_112576

Q9H299

Human

MSGLRVY STSVTGSREI KSQQSEVTRI LDGKRIQYQL VDISQDNALR DEMRALAGNP KATPPQIVNG DQYCGDYELF VEAVEQNTLQ EFLKLA