Recombinant humaan SHC1-eiwit

SHC1 (SHC-transforming protein 1) belongs to the SH2 domain family. SHC1 has been found to be important in the regulation of apoptosis and drug resistance in mammalian cells. It is a signaling adapter that couples activated growth factor receptors to signaling pathway. SHC1 expressed in neural stem cells but absent in mature neurons. Recombinant human SHC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

SHC-transforming protein 1, FLJ26504, SH2 domain protein C1, SHC, SHC-transforming protein 3, p66, MGC98812, SHCA, Src homology 2 domain containing transforming protein C1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

53.8 kDa (494aa)

SHC1, SHC-transforming protein 1, FLJ26504, SH2 domain protein C1, SHC, SHC-transforming protein 3, p66, MGC98812, SHCA, Src homology 2 domain containing transforming protein C1, ATGP1128-10 µg, ATGP1128-20 µg, ATGP1128-50 µg, ATGP1128-100 µg, ATGP1128-250 µg, ATGP1128-500 µg, ATGP1128-1 mg, ATGP1128-10, ATGP1128-20, ATGP1128-50, ATGP1128-100, ATGP1128-250, ATGP1128-500, ATGP1128-1

Debnath J., et al. (2010) Oncogene. 29 (41) :5556-8.; ; Zhou M.-M., et al. (1995) Proc. Natl. Acad. Sci. u.S.A. 92:7784-7788

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl, 0.1mM PMSF

Neuroscience

NP_003020

P29353

Human

MNKLSGGGGR RTRVEGGQLG GEEWTRHGSF VNKPTRGWLH PNDKVMGPGV SYLVRYMGCV EVLQSMRALD FNTRTQVTRE AISLVCEAVP GAKGATRRRK PCSRPLSSIL GRSNLKFAGM PITLTVSTSS LNLMAADCKQ IIANHHMQSI SFASGGDPDT AEYVAYVAKD PVNQRACHIL ECPEGLAQDV ISTIGQAFEL RFKQYLRNPP KLVTPHDRMA GFDGSAWDEE EEEPPDHQYY NDFPGKEPPL GGVVDMRLRE GAAPGAARPT APNAQTPSHL GATLPVGQPV GGDPEVRKQM PPPPPCPAGR ELFDDPSYVN VQNLDKARQA VGGAGPPNPA INGSAPRDLF DMKPFEDALR VPPPPQSVSM AEQLRGEPWF HGKLSRREAE ALLQLNGDFL VRESTTTPGQ YVLTGLQSGQ PKHLLLVDPE GVVRTKDHRF ESVSHLISYH MDNHLPIISA GSELCLQQPV ERKL