Recombinant humaan SHFM1-eiwit

SHFM1, as known as 26S proteasome complex subunit DSS1, has been proposed to be a candidate gene for the autosomal dominant form of the heterogeneous limb developmental disorder split hand/split foot malformation type 1. In addition, it has been shown to directly interact with BRCA2. It also may play a role in the completion of the cell cycle. Recombinant human SHFM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

26S proteasome complex subunit DSS1, DSS1, ECD, SEM1, SHFD1, Shfdg1, SHSF1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

10.7 kDa (93aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

SHFM1, 26S proteasome complex subunit DSS1, DSS1, ECD, SEM1, SHFD1, Shfdg1, SHSF1, ATGP2440-10 µg, ATGP2440-20 µg, ATGP2440-50 µg, ATGP2440-100 µg, ATGP2440-250 µg, ATGP2440-500 µg, ATGP2440-1 mg, ATGP2440-10, ATGP2440-20, ATGP2440-50, ATGP2440-100, ATGP2440-250, ATGP2440-500, ATGP2440-1

Bonache S. et al. (2013) J Cancer Res Clin Oncol. 139:529-32; ; Ma YY. et al. (2013) Carcinogenesis. 34:68-78.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Ubiquitination

NP_006295

P60896

Human

MGSSHHHHHH SSGLVPRGSH MGSMSEKKQP VDLGLLEEDD EFEEFPAEDW AGLDEDEDAH VWEDNWDDDN VEDDFSNQLR AELEKHGYKM ETS