Recombinant humaan SIAH1-eiwit

E3 ubiquitin-protein ligase SIAH1, also known as SIAH1, is a member of the seven in absentia homolog (SIAH) family. SIAH1 is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. SIAH1 contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. Recombinant human SIAH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

E3 ubiquitin-protein ligase SIAH1, SIAH1A

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.1 kDa (216aa) confirmed by MALDI-TOF

SIAH1, E3 ubiquitin-protein ligase SIAH1, SIAH1A, ATGP1669-10 µg, ATGP1669-20 µg, ATGP1669-50 µg, ATGP1669-100 µg, ATGP1669-250 µg, ATGP1669-500 µg, ATGP1669-1 mg, ATGP1669-10, ATGP1669-20, ATGP1669-50, ATGP1669-100, ATGP1669-250, ATGP1669-500, ATGP1669-1

Hu G., et al. (1999) Mol Cell Biol. 19: 724-732; ; Matsuzawas S., et al. (1998) EMBO J. 17: 2736-2747.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 40% glycerol,1mM DTT

Ubiquitination

NP_003022

Q8IUQ4

Human

VANSVLF PCKYASSGCE ITLPHTEKAD HEELCEFRPY SCPCPGASCK WQGSLDAVMP HLMHQHKSIT TLQGEDIVFL ATDINLPGAV DWVMMQSCFG FHFMLVLEKQ EKYDGHQQFF AIVQLIGTRK QAENFAYRLE LNGHRRRLTW EATPRSIHEG IATAIMNSDC LVFDTSIAQL FAENGNLGIN VTISMC