Recombinant humaan SIL1-eiwit

Nucleotide exchange factor, also known as SIL1, is a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Recombinant human SIL1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nucleotide exchange factor SIL1, BAP, MSS, uLG5

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

50 kDa (439aa)

SIL1, Nucleotide exchange factor SIL1, BAP, MSS, uLG5, ATGP1580-10 µg, ATGP1580-20 µg, ATGP1580-50 µg, ATGP1580-100 µg, ATGP1580-250 µg, ATGP1580-500 µg, ATGP1580-1 mg, ATGP1580-10, ATGP1580-20, ATGP1580-50, ATGP1580-100, ATGP1580-250, ATGP1580-500, ATGP1580-1

Chung KT., et al. (2002) J Biol Chem. 277 (49) :47557-63.; ; Senderek J., et al. (2005) Nat Genet. 37 (12) : 1312-4.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Heat Shock Proteins

NP_071909

Q9H173

Human

MHQNLKEFAL TNPEKSSTKE TERKETKAEE ELDAEVLEVF HPTHEWQALQ PGQAVPAGSH VRLNLQTGER EAKLQYEDKF RNNLKGKRLD INTNTYTSQD LKSALAKFKE GAEMESSKED KARQAEVKRL FRPIEELKKD FDELNVVIET DMQIMVRLIN KFNSSSSSLE EKIAALFDLE YYVHQMDNAQ DLLSFGGLQV VINGLNSTEP LVKEYAAFVL GAAFSSNPKV QVEAIEGGAL QKLLVILATE QPLTAKKKVL FALCSLLRHF PYAQRQFLKL GGLQVLRTLV QEKGTEVLAV RVVTLLYDLV TEKMFAEEEA ELTQEMSPEK LQQYRQVHLL PGLWEQGWCE ITAHLLALPE HDAREKVLQT LGVLLTTCRD RYRQDPQLGR TLASLQAEYQ VLASLELQDG EDEGYFQELL GSVNSLLKEL RLEHHHHHH