Recombinant humaan Sirtuïne 1/SIRT1-eiwit

SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. Alternative splicing results in multiple transcript variants. Recombinant human SIRT1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2, SIR2L1, Sirtuin, Silent mating type information regulation 2 homolog 1 (S. cerevisiae)

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

31.6 kDa (280aa)

Sirtuin1, SIRT1, Sirtuin 1, Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), SIR2L1, ATGP2625-10 µg, ATGP2625-20 µg, ATGP2625-50 µg, ATGP2625-100 µg, ATGP2625-250 µg, ATGP2625-500 µg, ATGP2625-1 mg, ATGP2625-10, ATGP2625-20, ATGP2625-50, ATGP2625-100, ATGP2625-250, ATGP2625-500, ATGP2625-1

Langley E., et al. (2002) EMBO J. 21:2383-2396.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Epigenomics (Transcription & Translation)

NP_036370

Q96EB6

Human

MGRDLYDDDD KDRWGSHMKK IIVLTGAGVS VSCGIPDFRS RDGIYARLAV DFPDLPDPQA MFDIEYFRKD PRPFFKFAKE IYPGQFQPSL CHKFIALSDK EGKLLRNYTQ NIDTLEQVAG IQRIIQCHGS FATASCLICK YKVDCEAVRG DIFNQVVPRC PRCPADEPLA IMKPEIVFFG ENLPEQFHRA MKYDKDEVDL LIVIGSSLKV RPVALIPSSI PHEVPQILIN REPLPHLHFD VELLGDCDVI INELCHRLGG