Recombinant humaan Sirtuïne 5/SIRT5 eiwit

SIRT5 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants Recombinant human SIRT5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NAD-dependent protein deacylase sirtuin-5 mitochondrial, Regulatory protein SIR2 homolog 5, SIR2-like protein 5, SIR2L5

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

32.5 kDa (300aa) confirmed by MALDI-TOF

Sirtuin5, SIRT5, sirtuin 5, SIR2L5, ATGP2434-10 µg, ATGP2434-20 µg, ATGP2434-50 µg, ATGP2434-100 µg, ATGP2434-250 µg, ATGP2434-500 µg, ATGP2434-1 mg, ATGP2434-10, ATGP2434-20, ATGP2434-50, ATGP2434-100, ATGP2434-250, ATGP2434-500, ATGP2434-1

Zhou, Y., et al. (2012) J. Biol. Chem. 287 (34), 28307-28314; ; Fischer, F., et al. (2012) PLoS ONE 7 (9), E45098

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Metabolism

NP_036373.1

Q9NXA8

Human

ARPSSSM ADFRKFFAKA KHIVIISGAG VSAESGVPTF RGAGGYWRKW QAQDLATPLA FAHNPSRVWE FYHYRREVMG SKEPNAGHRA IAECETRLGK QGRRVVVITQ NIDELHRKAG TKNLLEIHGS LFKTRCTSCG VVAENYKSPI CPALSGKGAP EPGTQDASIP VEKLPRCEEA GCGGLLRPHV VWFGENLDPA ILEEVDRELA HCDLCLVVGT SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFRFHFQ GPCGTTLPEA LACHENETVS