Recombinant humaan Sirtuïne2/SIRT2-eiwit

SIRT2 (NAD-dependent deacetylase sirtuin-2) is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIRT2 is a NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Recombinant human SIRT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NAD-dependent protein deacetylase sirtuin-2, NAD-dependent protein defatty-acylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIR2L2, Sirtuin, Silent mating type information regulation 2 homolog 2 (S. cerevisiae) cerevisiae)

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

41.6 kDa (372aa) confirmed by MALDI-TOF

Sirtuin2, SIRT2, NAD-dependent deacetylase sirtuin-2, SIR2, SIR2L, SIR2L2, ATGP0803-10 µg, ATGP0803-20 µg, ATGP0803-50 µg, ATGP0803-100 µg, ATGP0803-250 µg, ATGP0803-500 µg, ATGP0803-1 mg, ATGP0803-10, ATGP0803-20, ATGP0803-50, ATGP0803-100, ATGP0803-250, ATGP0803-500, ATGP0803-1

North B.J., et al. (2003) Mol. Cell 11:437-444; ; Dryden S.C., et al. (2003) Mol. Cell. Biol. 23:3173-3185

Kawaguchi M, et al. A Fluorescent Probe for Imaging Sirtuin Activity in Living Cells, Based on One-Step Cleavage of the Dabcyl Quencher. (Chembiochem. 2016) {https://pubmed.ncbi.nlm.nih.gov/27542094/}; ; Kawaguchi M, et al. Development of Peptide-Based Sirtuin Defatty-Acylase Inhibitors Identified by the Fluorescence Probe, SFP3, That Can Efficiently Measure Defatty-Acylase Activity of Sirtuin. (J Med Chem. 2019) {https://pubmed.ncbi.nlm.nih.gov/31117516/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 2mM DTT, 200 mM NaCl, 0.5mM EDTA

Cell Cycle

NP_085096

Q8IXJ6

Human

MDFLRNLFSQ TLSLGSQKER LLDELTLEGV ARYMQSERCR RVICLVGAGI STSAGIPDFR SPSTGLYDNL EKYHLPYPEA IFEISYFKKH PEPFFALAKE LYPGQFKPTI CHYFMRLLKD KGLLLRCYTQ NIDTLERIAG LEQEDLVEAH GTFYTSHCVS ASCRHEYPLS WMKEKIFSEV TPKCEDCQSL VKPDIVFFGE SLPARFFSCM QSDFLKVDLL LVMGTSLQVQ PFASLISKAP LSTPRLLINK EKAGQSDPFL GMIMGLGGGM DFDSKKAYRD VAWLGECDQG CLALAELLGW KKELEDLVRR EHASIDAQSG AGVPNPSTSA SPKKSPPPAK DEARTTEREK PQ