Recombinant humaan SIT1-eiwit

SIT1, also known as signaling threshold-regulating transmembrane adapter 1, negatively regulates TCR (T-cell antigen receptor) -mediated signaling in T-cells. This protein involved in positive selection of T-cells. Recombinant human SIT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Signaling threshold-regulating transmembrane adapter 1, SIT

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

16.9 kDa (156aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

SIT1, Signaling threshold-regulating transmembrane adapter 1, SIT, ATGP1209-10 µg, ATGP1209-20 µg, ATGP1209-50 µg, ATGP1209-100 µg, ATGP1209-250 µg, ATGP1209-500 µg, ATGP1209-1 mg, ATGP1209-10, ATGP1209-20, ATGP1209-50, ATGP1209-100, ATGP1209-250, ATGP1209-500, ATGP1209-1

Marie-Cardine A., et al. (1999) J. Exp. Med. 189:1181-1194; ; Pfrepper K., et al. (2001) Eur. J. Immunol. 31:1825-1836

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Immunology

NP_055265.1

Q9Y3P8

Human

HLSQWTRGR SRSHPGQGRS GESVEEVPLY GNLHYLQTGR LSQDPEPDQQ DPTLGGPARA AEEVMCYTSL QLRPPQGRIP GPGTPVKYSE VVLDSEPKSQ ASGPEPELYA SVCAQTRRAR ASFPDQAYAN SQPAAS