Recombinant humaan SIX6-eiwit

SIX6, also as known as OPTX2 and SIX9, belongs to the SIX/Sine oculis homeobox family contains 1 homeobox DNA-binding domain. The SIX homeobox protein is crucial in embryonic development by providing necessary instructions for the formation of the forebrain and eye development. This protein expressed in the developing and adult retina, hypothalamic and the pituitary regions. Defects in SIX6 are the cause of microphthalmia isolated with cataract type 2. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Recombinant human SIX6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

SIX homeobox 6, MCOPCT2, Six9

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.1 kDa (269aa) confirmed by MALDI-TOF

SIX6, SIX homeobox 6, MCOPCT2, Six9, ATGP1758-10 µg, ATGP1758-20 µg, ATGP1758-50 µg, ATGP1758-100 µg, ATGP1758-250 µg, ATGP1758-500 µg, ATGP1758-1 mg, ATGP1758-10, ATGP1758-20, ATGP1758-50, ATGP1758-100, ATGP1758-250, ATGP1758-500, ATGP1758-1

Gallardo M.E., et al. (2004) Am. J. Med. Genet. A 129:92-94; ; Lopez-Rios J., et al. (1999) Mech. Dev. 83:155-159

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 5mM DTT, 2mM EDTA

Epigenomics (Transcription & Translation)

AAH69413

O95475

Human

MFQLPIL NFSPQQVAGV CETLEESGDV ERLGRFLWSL PVAPAACEAL NKNESVLRAR AIVAFHGGNY RELYHILENH KFTKESHAKL QALWLEAHYQ EAEKLRGRPL GPVDKYRVRK KFPLPRTIWD GEQKTHCFKE RTRNLLREWY LQDPYPNPSK KRELAQATGL TPTQVGNWFK NRRQRDRAAA AKNRLQQQVL SQGSGRALRA EGDGTPEVLG VATSPAASLS SKAATSAISI TSSDSECDI