Recombinant humaan SKA1-eiwit

SKA1, as known as spindle and kinetochore-associated protein 1, is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules. Recombinant human SKA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Spindle and kinetochore-associated protein 1, Spindle and kinetochore-associated protein 1, C18orf24

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

31.9 kDa (278aa) confirmed by MALDI-TOF

SKA1, Spindle and kinetochore-associated protein 1, Spindle and kinetochore-associated protein 1, C18orf24, ATGP2596-10 µg, ATGP2596-20 µg, ATGP2596-50 µg, ATGP2596-100 µg, ATGP2596-250 µg, ATGP2596-500 µg, ATGP2596-1 mg, ATGP2596-10, ATGP2596-20, ATGP2596-50, ATGP2596-100, ATGP2596-250, ATGP2596-500, ATGP2596-1

Ota T. et al. (2004) Nat Genet. 36:40-45; ; Hanisch A. et al. (2006) EMBO J. 25: 5504-5515.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Cell Cycle

NP_001034624

Q96BD8

Human

MGSSHHHHHH SSGLVPRGSH MGSMASSDLE QLCSHVNEKI GNIKKTLSLR NCGQEPTLKT VLNKIGDEII VINELLNKLE LEIQYQEQTN NSLKELCESL EEDYKDIEHL KENVPSHLPQ VTVTQSCVKG SDLDPEEPIK VEEPEPVKKP PKEQRSIKEM PFITCDEFNG VPSYMKSRLT YNQINDVIKE INKAVISKYK ILHQPKKSMN SVTRNLYHRF IDEETKDTKG RYFIVEADIK EFTTLKADKK FHVLLNILRH CRRLSEVRGG GLTRYVIT