Recombinant humaan SKP1-eiwit

S-phase kinase-associated protein 1, also known as SKP1, is an F-box protein which functions as a substrate recognition component of the SCF ubiquitin ligase complex. It binds to proteins containing an F-box motif, such as cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin dependent proteolysis. It is also involved in the control of beta-catenin levels and the activity of beta-catenin dependent TCF transcription factors. Recombinant human SKP1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

S-phase kinase-associated protein 1 isoform a, EMC19, MGC34403, OCP-II, OCP2, p19A, SKP1A, TCEB1L, S-phase kinase-associated protein 1 isoform a Cyclin A/CDK2 associated p19, Cyclin A/CDK2 associated protein p19, MGC34403, OCP 2, OCP II, OCP II protein, OCPII, Organ of Corti protein 2, p19skp1, RNA polymerase II elongation factor like protein, RNA polymerase II elongation factor like protein OCP2, S phase kinase associated protein 1, S phase kinase associated protein 1A, S phase kinase associated protein 1A p19A, SIII, Skp 1, SKP 1A, Transcription elongation factor B, Transcription Elongation Factor B 1 like, Transcription elongation factor B SIII polypeptide 1 like.

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

18 kDa (160aa) confirmed by MALDI-TOF

SKP1, S-phase kinase-associated protein 1 isoform a, EMC19, MGC34403, OCP-II, OCP2, p19A, SKP1A, TCEB1L, S-phase kinase-associated protein 1 isoform a Cyclin A/CDK2 associated p19, Cyclin A/CDK2 associated protein p19, MGC34403, OCP 2, OCP II, OCP II protein, OCPII, Organ of Corti protein 2, p19skp1, RNA polymerase II elongation factor like protein, RNA polymerase II elongation factor like protein OCP2, S phase kinase associated protein 1, S phase kinase associated protein 1A, S phase kinase associated protein 1A p19A, SIII, Skp 1, SKP 1A, Transcription elongation factor B, Transcription Elongation Factor B 1 like, Transcription elongation factor B SIII polypeptide 1 like., ATGP0430-10 µg, ATGP0430-20 µg, ATGP0430-50 µg, ATGP0430-100 µg, ATGP0430-250 µg, ATGP0430-500 µg, ATGP0430-1 mg, ATGP0430-10, ATGP0430-20, ATGP0430-50, ATGP0430-100, ATGP0430-250, ATGP0430-500, ATGP0430-1

Cenciarelli C., et al. (1999) Curr Biol. 9 (20) : 1177-9.; ; Min KW., et al. (2003) J Biol Chem. 278 (18) :15905-10.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM NaCl, 10% glycerol

Ubiquitination

NP_008861.2

P63208

Human

MPSIKLQSSD GEIFEVDVEI AKQSVTIKTM LEDLGMDDEG DDDPVPLPNV NAAILKKVIQ WCTHHKDDPP PPEDDENKEK RTDDIPVWDQ EFLKVDQGTL FELILAANYL DIKGLLDVTC KTVANMIKGK TPEEIRKTFN IKNDFTEEEE AQVGSTQFCL