Recombinant humaan SLD5/GINS4-eiwit

GINS4 plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA. Recombinant human GINS4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA replication complex GINS protein SLD5, GINS complex subunit 4, GINS4, SLD5

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.2 kDa (243aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

GINS4, DNA replication complex GINS protein SLD5, SLD5, GINS complex subunit 4, ATGP1792-10 µg, ATGP1792-20 µg, ATGP1792-50 µg, ATGP1792-100 µg, ATGP1792-250 µg, ATGP1792-500 µg, ATGP1792-1 mg, ATGP1792-10, ATGP1792-20, ATGP1792-50, ATGP1792-100, ATGP1792-250, ATGP1792-500, ATGP1792-1

Kamada K., et al. (2007) Nat. Struct. Mol. Biol. 14:388-396; ; Chang Y.P., et al. (2007) Proc. Natl. Acad. Sci. u.S.A. 104:12685-12690;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_115712

Q9BRT9

Human

MGSSHHHHHH SSGLVPRGSH MTEEVDFLGQ DSDGGSEEVV LTPAELIERL EQAWMNEKFA PELLESKPEI VECVMEQLEH MEENLRRAKR EDLKVSIHQM EMERIRYVLS SYLRCRLMKI EKFFPHVLEK EKTRPEGEPS SLSPEELAFA REFMANTESY LKNVALKHMP PNLQKVDLFR AVPKPDLDSY VFLRVRERQE NILVEPDTDE QRDYVIDLEK GSQHLIRYKT IAPLVASGAV QLI