Recombinant humaan SLPI-eiwit

SLPI is a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes; the protein is also thought to have broad-spectrum anti-biotic activity. Recombinant human SLPI, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Secretory leukocyte peptidase inhibitor, Antileukoproteinase, ALK1, ALP, BLPI, HuSI, HuSI-I, MPI, WAP4, WFDC4, secretory leukocyte peptidase inhibitor, ALK 1, Antileukoproteinase 1, Antileukoproteinase1, HuSI 1, HuSI I, Protease inhibitor WAP4, Secretory leukocyte protease inhibitor, Seminal proteinase inhibitor, WAP four disulfide core domain protein 4, WAP 4, WFDC 4

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

14 kDa (128aa) confirmed by MALDI-TOF

SLPI, secretory leukocyte peptidase inhibitor, Antileukoproteinase, ALK1, ALP, BLPI, HuSI, HuSI-I, MPI, WAP4, WFDC4, secretory leukocyte peptidase inhibitor, ALK 1, Antileukoproteinase 1, Antileukoproteinase1, HuSI 1, HuSI I, Protease inhibitor WAP4, Secretory leukocyte protease inhibitor, Seminal proteinase inhibitor, WAP four disulfide core domain protein 4, WAP 4, WFDC 4, ATGP0599-10 µg, ATGP0599-20 µg, ATGP0599-50 µg, ATGP0599-100 µg, ATGP0599-250 µg, ATGP0599-500 µg, ATGP0599-1 mg, ATGP0599-10, ATGP0599-20, ATGP0599-50, ATGP0599-100, ATGP0599-250, ATGP0599-500, ATGP0599-1

Rasool N., et al. (2010) Clin Cancer Res. 16 (2) :600-9; ; King AE., et al. (2009) Hum Reprod. 24 (3) :679-86.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100 mM NaCl

Immunology

NP_003055.1

P03973

Human

SGKSFKAGV CPPKKSAQCL RYKKPECQSD WQCPGKKRCC PDTCGIKCLD PVDTPNPTRR KPGKCPVTYG QCLMLNPPNF CEMDGQCKRD LKCCMGMCGK SCVSPVKA