Recombinant humaan Smad3-eiwit

SMAD3, also known as mothers against decapentaplegic homolog 3, belongs to the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. Small moleucule inhibitors of Smad3 may have tremendous clinical potential in the treatment of pathological fibrotic diseases. Recombinant SMAD3 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Mothers against decapentaplegic homolog 3, HSPC193, HsT17436, JV15-2, MADH3, Mothers against decapentaplegic homolog 3 DKFZP586N0721, DKFZp686J10186, hMAD 3, hSMAD3, MAD (mothers against decapentaplegic Drosophila) homolog 3, HST17436, JV15 2, JV152, MAD3, MADH 3, MGC60396, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

50.2 kDa (445aa) confirmed by MALDI-TOF

Smad3, ATGP0421-10 µg, ATGP0421-20 µg, ATGP0421-50 µg, ATGP0421-100 µg, ATGP0421-250 µg, ATGP0421-500 µg, ATGP0421-1 mg, ATGP0421-10, ATGP0421-20, ATGP0421-50, ATGP0421-100, ATGP0421-250, ATGP0421-500, ATGP0421-1

Zhang Y., et al. (1996) Nature. 383 (6596) :168-72.; ; Wang H., et al. (2005) J Biol Chem. 280 (7) :5154-62.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl and 1mM DTT

Epigenomics (Transcription & Translation)

NP_005893

P84022

Human

MSSILPFTPP IVKRLLGWKK GEQNGQEEKW CEKAVKSLVK KLKKTGQLDE LEKAITTQNV NTKCITIPRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELRAMELCE FAFNMKKDEV CVNPYHYQRV ETPVLPPVLV PRHTEIPAEF PPLDDYSHSI PENTNFPAGI EPQSNIPETP PPGYLSEDGE TSDHQMNHSM DAGSPNLSPN PMSPAHNNLD LQPVTYCEPA FWCSISYYEL NQRVGETFHA SQPSMTVDGF TDPSNSERFC LGLLSNVNRN AAVELTRRHI GRGVRLYYIG GEVFAECLSD SAIFVQSPNC NQRYGWHPAT VCKIPPGCNL KIFNNQEFAA LLAQSVNQGF EAVYQLTRMC TIRMSFVKGW GAEYRRQTVT STPCWIELHL NGPLQWLDKV LTQMGSPSIR CSSVS