Recombinant humaan SMCP-eiwit

Sperm mitochondria differ in morphology and subcellular localization from those of somatic cells. They are elongated, flattened, and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum. SMCP localizes to the capsule associated with the mitochondrial outer membranes and is thought to function in the organization and stabilization of the helical structure of the sperm's mitochondrial sheath. Recombinant human SMCP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sperm mitochondria-associated cysteine-rich protein, HSMCSGEN1, MCS, MCSP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

15.2 kDa (139aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

SMCP, Sperm mitochondria-associated cysteine-rich protein, HSMCSGEN1, MCS, MCSP, ATGP2354-10 µg, ATGP2354-20 µg, ATGP2354-50 µg, ATGP2354-100 µg, ATGP2354-250 µg, ATGP2354-500 µg, ATGP2354-1 mg, ATGP2354-10, ATGP2354-20, ATGP2354-50, ATGP2354-100, ATGP2354-250, ATGP2354-500, ATGP2354-1

Aho H., et al. (1996) Genomics. 32:184-190; ; Hawthorne S.K., et al. (2006) Genomics. 87:382-391

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM EDTA, 0.1mM PMSF

Development

NP_109588

P49901

Human

MGSSHHHHHH SSGLVPRGSH MGSMCDQTKH SKCCPAKGNQ CCPPQQNQCC QSKGNQCCPP KQNQCCQPKG SQCCPPKHNH CCQPKPPCCI QARCCGLETK PEVSPLNMES EPNSPQTQDK GCQTQQQPHS PQNESRPSK