Recombinant humaan SMNDC1-eiwit

SMNDC1 (survival motor neuron domain containing 1) is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (Survival Motor Neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. Mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. Recombinant human SMNDC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Survival of motor neuron-related-splicing factor 30, Survival of motor neuron-related-splicing factor 30

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.9 kDa (258aa) confirmed by MALDI-TOF

SMNDC1, Survival of motor neuron-related-splicing factor 30, Survival of motor neuron-related-splicing factor 30, ATGP0807-10 µg, ATGP0807-20 µg, ATGP0807-50 µg, ATGP0807-100 µg, ATGP0807-250 µg, ATGP0807-500 µg, ATGP0807-1 mg, ATGP0807-10, ATGP0807-20, ATGP0807-50, ATGP0807-100, ATGP0807-250, ATGP0807-500, ATGP0807-1

Meister G., et al. (2001) EMBO J. 20:2304-2314; ; Talbot K., et al. (1998) Hum. Mol. Genet. 7:2149-2156

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl

Apoptosis

NP_005862

O75940

Human

MGSSHHHHHH SSGLVPRGSH MSEDLAKQLA SYKAQLQQVE AALSGNGENE DLLKLKKDLQ EVIELTKDLL STQPSETLAS SDSFASTQPT HSWKVGDKCM AVWSEDGQCY EAEIEEIDEE NGTAAITFAG YGNAEVTPLL NLKPVEEGRK AKEDSGNKPM SKKEMIAQQR EYKKKKALKK AQRIKELEQE REDQKVKWQQ FNNRAYSKNK KGQVKRSIFA SPESVTGKVG VGTCGIADKP MTQYQDTSKY NVRHLMPQ