Recombinant humaan SMUG1-eiwit

Single-strand-selective monofunctional uracil-DNA glycosylase, also known as SMuG1, is a enzyme responsible for recognizing base lesions in the genome and initiating base excision DNA repair. This protein acts as a monofunctional DNA glycosylase specific for uracil (u) residues in DNA and has a preference for single-stranded DNA substrates. Recombinant human SMuG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Single-strand-selective monofunctional uracil-DNA glycosylase, FDG, HMuDG, uNG3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

32.3 kDa (293aa) confirmed by MALDI-TOF

SMUG1, Single-strand-selective monofunctional uracil-DNA glycosylase, FDG, HMuDG, uNG3, ATGP1848-10 µg, ATGP1848-20 µg, ATGP1848-50 µg, ATGP1848-100 µg, ATGP1848-250 µg, ATGP1848-500 µg, ATGP1848-1 mg, ATGP1848-10, ATGP1848-20, ATGP1848-50, ATGP1848-100, ATGP1848-250, ATGP1848-500, ATGP1848-1

Masaoka A., et al. (2003) Biochemistry. 42:5003-5012; ; Boorstein R.J., et al. (2001) J. Biol. Chem. 276:41991-41997;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_055126

Q53HV7

Human

MGSSHHHHHH SSGLVPRGSH MGSMPQAFLL GSIHEPAGAL MEPQPCPGSL AESFLEEELR LNAELSQLQF SEPVGIIYNP VEYAWEPHRN YVTRYCQGPK EVLFLGMNPG PFGMAQTGVP FGEVSMVRDW LGIVGPVLTP PQEHPKRPVL GLECPQSEVS GARFWGFFRN LCGQPEVFFH HCFVHNLCPL LFLAPSGRNL TPAELPAKQR EQLLGICDAA LCRQVQLLGV RLVVGVGRLA EQRARRALAG LMPEVQVEGL LHPSPRNPQA NKGWEAVAKE RLNELGLLPL LLK