Recombinant humaan SNRPE-eiwit

SNRPE, also known as SME, aids in the cytoplasmic construction of the usnRNPs by binding to a conserved Sm site on usnRNA and forming a stable snRNP core complex. As a core protein to usnRNP, the SNRPE associates with the entire u family of snRNAs including u1-u6. SNRPE also interacts with DDX20 and Small nuclear ribonucleoprotein polypeptide F. Recombinant human SNRPE protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Small nuclear ribonucleoprotein E, B-raf, Sm-E, SME

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

12.9 kDa (112aa) confirmed by MALDI-TOF

SNRPE, Small nuclear ribonucleoprotein E, B-raf, Sm-E, SME, ATGP0919-10 µg, ATGP0919-20 µg, ATGP0919-50 µg, ATGP0919-100 µg, ATGP0919-250 µg, ATGP0919-500 µg, ATGP0919-1 mg, ATGP0919-10, ATGP0919-20, ATGP0919-50, ATGP0919-100, ATGP0919-250, ATGP0919-500, ATGP0919-1

Campion Y., et al. (2010) EMBO J. 29 (11) :1817-29.; ; Dong X., et al. (2009) Nature. 458 (7242) :1136-41.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol

Other

NP_003085

P62304

Human

MGSSHHHHHH SSGLVPRGSH MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQSV SN