Recombinant humaan SNRPF-eiwit

Small nuclear ribonucleoprotein polypeptide F, also known SNRPF, belongs to the snRNP Sm proteins family. There are at least seven isoforms, E, F, G, D1, D2, D3 and B/B. This class of common proteins plays an essential role in the biogenesis of the snRNPs. In addition, these proteins represent the major targets for the so-called anti-Sm auto-antibodies which are diagnostic for systemic lupus erythematosus (SLE) . SNRPF aid in the cytoplasmic construction of the usnRNPs by binding to aconserved Sm site on usnRNA and forming a stable snRNP core complex. Recombinant human SNRPF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Small nuclear ribonucleoprotein polypeptide F, Sm-F, SMF, PBSCF

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

11.8 kDa (106aa) confirmed by MALDI-TOF

SNRPF, Small nuclear ribonucleoprotein polypeptide F, Sm-F, SMF, PBSCF, ATGP0820-10 µg, ATGP0820-20 µg, ATGP0820-50 µg, ATGP0820-100 µg, ATGP0820-250 µg, ATGP0820-500 µg, ATGP0820-1 mg, ATGP0820-10, ATGP0820-20, ATGP0820-50, ATGP0820-100, ATGP0820-250, ATGP0820-500, ATGP0820-1

Brahms H., et al. (1997) Arthritis Rheum. 40:372-682.; ; Raker V A., et al. (1996) EMBO J. 15:2256-2269.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 1mM EDTA

Metabolism

NP_003086

P62306

Human

MGSSHHHHHH SSGLVPRGSH MSLPLNPKPF LNGLTGKPVM VKLKWGMEYK GYLVSVDGYM NMQLANTEEY IDGALSGHLG EVLIRCNNVL YIRGVEEEEE DGEMRE