Recombinant humaan SNTN-eiwit

SNTN, also known as sentan, belongs to the S-100 family. SNTN is shown to localize exclusively to the bridging structure between the cell membrane and peripheral singlet microtubules, which specifically exists in the narrowed distal portion of cilia. Exogenously expressed sentan showed affinity for the membrane protrusions, and a protein-lipid binding assay revealed that sentan bound to phosphatidylserine. These findings suggest that sentan is the first molecular component of the ciliary tip to bridge the cell membrane and peripheral singlet microtubules, making the distal portion of the cilia narrow and stiff to allow for better airway clearance or ovum transport. Recombinant human SNTN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Sentan, S100A1L, S100AL

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

18.6 kDa (167aa) confirmed by MALDI-TOF

SNTN, Sentan, S100A1L, S100AL, ATGP1043-10 µg, ATGP1043-20 µg, ATGP1043-50 µg, ATGP1043-100 µg, ATGP1043-250 µg, ATGP1043-500 µg, ATGP1043-1 mg, ATGP1043-10, ATGP1043-20, ATGP1043-50, ATGP1043-100, ATGP1043-250, ATGP1043-500, ATGP1043-1

Muzny D.M., et al. (2006) Nature. 440:1194-1198; ; Kubo A., et al. (2008) Mol. Biol. Cell 19:5338-5346

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.15M NaCl, 1mM DTT

Signal Transduction

NP_001074006

A6NMZ2

Human

MGSSHHHHHH SSGLVPRGSH MGGCMHSTQD KSLHLEGDPN PSAAPTSTCA PRKMPKRISI SKQLASVKAL RKCSDLEKAI ATTALIFRNS SDSDGKLEKA IAKDLLQTQF RNFAEGQETK PKYREILSEL DEHTENKLDF EDFMILLLSI TVMSDLLQNI RNVKIMK