Recombinant humaan SNURF-eiwit

SNuRF is a highly basic protein localized to the nucleus. The evolutionarily constrained open reading frame is found on a bicistronic transcript which has a downstream ORF encoding the small nuclear ribonucleoprotein polypeptide N. The upstream coding region utilizes the first three exons of the transcript, a region that has been identified as an imprinting center. Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region but the full-length nature of these transcripts has not been determined. An alternate exon has been identified that substitutes for exon 4 and leads to a truncated, monocistronic transcript. Alternative splicing or deletion caused by a translocation event in the 5' uTR or coding region of this gene leads to Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure. The function of this protein is not yet known. Recombinant human SNuRF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

SNRPN upstream reading frame protein, Small nuclear ring finger protein

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

10.8 kDa (94aa) confirmed by MALDI-TOF

SNURF, SNRPN upstream reading frame protein, Small nuclear ring finger protein, ATGP2528-10 µg, ATGP2528-20 µg, ATGP2528-50 µg, ATGP2528-100 µg, ATGP2528-250 µg, ATGP2528-500 µg, ATGP2528-1 mg, ATGP2528-10, ATGP2528-20, ATGP2528-50, ATGP2528-100, ATGP2528-250, ATGP2528-500, ATGP2528-1

Runte M, Kroisel PM et al. (2004) . Hum Genet. 114 (6) :553-61.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 40% glycerol, 1mM DTT

Other

NP_073715

Q9Y675

Human

MGSSHHHHHH SSGLVPRGSH MGSMERARDR LHLRRTTEQH VPEVEVQVKR RRTASLSNQE CQLYPRRSQQ QQVPVVDFQA ELRQAFLAET PRGG