Recombinant humaan Snurportine 1/SNUPN-eiwit

SNUPN, also known as KPNBL or RNUT1, is a nuclear import adaptor protein belonging to the Snurportin family. Localizing to the cytoplasm and nucleus, Snurportin-1 contains an N-terminal IBB domain and a trimethylguanosine (m3G) -cap binding domain. It specifically binds the terminal 2, 2, 7-m3G-cap at the 5' end of u snRNPs and functions to transport u snRNPs into the nucleus via an association with Importin beta. Recombinant human Snurportin-1 protien, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

RNA U transporter 1, SNURPORTIN-1, RNUT1, SPN1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

43.3 (380aa) confirmed by MALDI-TOF

SNUPN, Snurportin1, KPNBL, RNuT1, Snurportin1, SPN1, Snurportin1, ATGP0640-10 µg, ATGP0640-20 µg, ATGP0640-50 µg, ATGP0640-100 µg, ATGP0640-250 µg, ATGP0640-500 µg, ATGP0640-1 mg, ATGP0640-10, ATGP0640-20, ATGP0640-50, ATGP0640-100, ATGP0640-250, ATGP0640-500, ATGP0640-1

Huber J., et al. (1998) EMBO J. 17 (14) :4114-26.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Adhesion & Movement

NP_001036053

O95149

Human

MEELSQALAS SFSVSQDLNS TAAPHPRLSQ YKSKYSSLEQ SERRRRLLEL QKSKRLDYVN HARRLAEDDW TGMESEEENK KDDEEMDIDT VKKLPKHYAN QLMLSEWLID VPSDLGQEWI VVVCPVGKRA LIVASRGSTS AYTKSGYCVN RFSSLLPGGN RRNSTAKDYT ILDCIYNEVN QTYYVLDVMC WRGHPFYDCQ TDFRFYWMHS KLPEEEGLGE KTKLNPFKFV GLKNFPCTPE SLCDVLSMDF PFEVDGLLFY HKQTHYSPGS TPLVGWLRPY MVSDVLGVAV PAGPLTTKPD YAGHQLQQIM EHKKSQKEGM KEKLTHKASE NGHYELEHLS TPKLKGSSHS PDHPGCLMEN