Recombinant humaan SNX5-eiwit

SNX5 also known as sorting nexin-5, is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein is a component of the mammalian retromer complex, which facilitates cargo retrieval from endosomes to the trans-Golgi network. It has also been shown to bind to the Fanconi anemia, complementation group A protein. Recombinant human SNX5, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sorting nexin-5 isoform a, Sorting nexin 5, snx 5

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

49.2 kDa (427aa) confirmed by MALDI-TOF

SNX5, Sorting nexin-5 isoform a, Sorting nexin 5, snx 5, ATGP3065-10 µg, ATGP3065-20 µg, ATGP3065-50 µg, ATGP3065-100 µg, ATGP3065-250 µg, ATGP3065-500 µg, ATGP3065-1 mg, ATGP3065-10, ATGP3065-20, ATGP3065-50, ATGP3065-100, ATGP3065-250, ATGP3065-500, ATGP3065-1

Wassmer T., et al. (2007) J Cell Sci. 120:45-54; ; Otsuki T., et al. (1999) Biochem Biophys Res Commun. 265:630-635.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Signal Transduction

NP_689413

Q9Y5X3

Human

MGSSHHHHHH SSGLVPRGSH MGSMAAVPEL LQQQEEDRSK LRSVSVDLNV DPSLQIDIPD ALSERDKVKF TVHTKTTLPT FQSPEFSVTR QHEDFVWLHD TLIETTDYAG LIIPPAPTKP DFDGPREKMQ KLGEGEGSMT KEEFAKMKQE LEAEYLAVFK KTVSSHEVFL QRLSSHPVLS KDRNFHVFLE YDQDLSVRRK NTKEMFGGFF KSVVKSADEV LFTGVKEVDD FFEQEKNFLI NYYNRIKDSC VKADKMTRSH KNVADDYIHT AACLHSLALE EPTVIKKYLL KVAELFEKLR KVEGRVSSDE DLKLTELLRY YMLNIEAAKD LLYRRTKALI DYENSNKALD KARLKSKDVK LAEAHQQECC QKFEQLSESA KEELINFKRK RVAAFRKNLI EMSELEIKHA RNNVSLLQSC IDLFKNN