Recombinant humaan Sonic Hedgehog/Shh (C24II) eiwit

SHH, also known as Sonic hedgehog protein, is one of three proteins in the mammalian signaling pathway family called hedgehog. It plays a key role in regulating vertebrate organogenesis, such as in the growth of digits on limbs and organization of the brain. It controls cell division of adult stem cells and has been implicated in development of some cancers. Recombinant human SHH was expressed in E. coli and purified by using conventional chromatography techniques.

Sonic hedgehog protein, Sonic hedgehog protein, HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

19.8 kDa (176aa) confirmed by MALDI-TOF

Sonic Hedgehog, Shh (C24II), Sonic hedgehog protein, Sonic hedgehog protein, HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS, ATGP3037-10 µg, ATGP3037-20 µg, ATGP3037-50 µg, ATGP3037-100 µg, ATGP3037-250 µg, ATGP3037-500 µg, ATGP3037-1 mg, ATGP3037-10, ATGP3037-20, ATGP3037-50, ATGP3037-100, ATGP3037-250, ATGP3037-500, ATGP3037-1

Richieri-Costa A., et al. (2006) Am. J. Med. Genet. A 140:2587-2593.; ; El-Jaick K.B., et al (2005) Am. J. Med. Genet. A 136:345-345.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Epigenomics (Transcription & Translation)

NP_000184

Q15465

Human

MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG